Designed for Bernstein Lab alumni ®.
1. Further studies will be needed to assess translational opportunities in GBM and to evaluate the relative merits of hierarchical and plasticity-based cellular models across the diverse spectrum of human malignancies.
2. Our methods should facilitate future studies of many other loci whose size, repetitive nature and/or allele-specific regulation hinder contemporary tools.
3. Further computational and experimental innovations are needed to scale these approaches and further illuminate the syntax of human regulatory genomics.
4. Conversely, disruption of chromosomal topology and oncogene insulation may be more generally relevant to methylator phenotypes observed in colorectal and renal cell carcinomas, leukaemia and other malignancies
5. Regardless, continued dissection of the reprogramming process promises for a comprehensive identification of a sufficient factor set for complete and safe somatic to pluripotent reprogramming
6. The single-molecule assay that we describe here requires little starting material and is highly scalable, given that many millions or even billions of nucleosomes may be decoded and sequenced in an automated imaging run.
